The General University Hospital in Prague is one of the largest facilities of its kind in the Czech Republic. The VFN is one of the Czech Republic's top institutions providing basic, specialized and superspecialized care for the citizens of the Prague and Central Bohemia regions, and in some fields specialized centers have nationwide coverage. The hospital also provides basic diagnostic and curative care for the residents of Prague 1, 2 and Prague 4.

The General Teaching Hospital (VFN) provides full-scale medical care, with the exception of burn medicine, plastic surgery, specialized neurosurgery and the treatment of infectious diseases. At the same time, it operates a comprehensive pharmacy service including technologically demanding preparations of cytostatics and sterile medicinal products.